Micronuclei Induction in AG01522 Cells is Independent of Temperature and Linear Energy Transfer

The bystander effect describes radiation-induced biological effects in nonirradiated cells that have received signals from irradiated cells. In a co-culture experiment, the bystander signaling is proposed to occur via the medium. Using a co-culture setup, the work in this thesis investigates the effects of temperature as an experimental parameter and linear energy transfer (LET) dependence on the bystander effect Using the micronucleus assay and primary human AG01522 fibroblast cells co-cultured as both the target and bystander cells, the incidence of micronuclei in both X-ray irradiated and alpha particle irradiated bystander experiments were -2 fold over control averages. In the temperature experiment, there were no significant differences between bystander cells cocultured with cold (40C) target cells and those co-cultured with warm control target cells. These results have shown, for AGO1 522 fibroblasts, that the bystander effect is independent of temperature and LET. Thesis Supervisor. Jacquelyn C. Yanch, Ph.D. Title: Professor of Nuclear Science and Engineering